Part:BBa_K5086115
hsa-mir-21_3DDSD_s
The hsa-mir-21_3DDSD_s (BBa_K5086115) is formed by the combination of hsa-mir-21_3DDSD (BBa_K5086025), hsa-mir-21_target (BBa_K5086002), and three wt (BBa_K5086017) chains. Among them, the sequence at the 5' end of hsa-mir-21_3DDSD is successively connected to the hsa-mir-21_target and the three wt chains in a complementary pairing, and the sequence at its 3' end is complementary to the Universalness_h (BBa_K5086009) chain, thus hsa- mir-21_3DDSD_s can bind specifically to the Universalness_h strand and thus complete the recognition of the input signal.
DNA polymerase polymerizes DNA in the 5'-3' direction starting from the 3' end of the target hsa-mir-21_Rep. During DNA polymerization by Bst DNA polymerase, the wt strand is replaced and released by the newly synthesized daughter strand, which also releases a DNA single strand with the same sequence as the target strand. At the same time, a single strand of DNA with the same sequence as the target strand (U to T) will be released, which will serve as the target to undergo the first step of the polymerase-mediated strand displacement reaction again, realizing the DDSD self-enhancement process, and the wt strand will be involved in the subsequent reaction to produce the fluorescence report.
The reaction was verified by electrophoresis as well as a fluorescence profile:
Fig.electropherogram
Fig.fluorescence plot
Sequence and Features
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