Part:BBa_K5086111
hsa-mir-21_2DDSD_s
The hsa-mir-21_2DDSD_s (BBa_K5086111) is formed by the combination of hsa-mir-21_2DDSD (BBa_K5086021), hsa-mir-21_target (BBa_K5086002), and two wt (BBa_K5086017) chains. Among them, the sequence at the 5' end of hsa-mir-21_2DDSD is successively complementary pair-joined to the hsa-mir-21_target and the two wt strands, and the sequence at its 3' end is complementary to the Universalness_h (BBa_K5086009) strand, thus hsa- mir-21_2DDSD_s can bind specifically to the Universalness_h strand and thus complete the recognition of the input signal. DNA polymerase polymerizes DNA in the 5'-3' direction starting from the 3' end of the target hsa-mir-21_Rep. During DNA polymerization by Bst DNA polymerase, the wt strand is displaced and released by the newly synthesized daughter strand, and a DNA single strand with sequence identical to that of the target strand is also released. At the same time, a single strand of DNA with the same sequence as the target strand (U to T) will be released, which will serve as the target to undergo the first step of the polymerase-mediated strand displacement reaction again, realizing the DDSD self-enhancement process, and the wt strand will be involved in the subsequent reaction to produce the fluorescence report.
Sequence and Features
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