Part:BBa_K5086110
hsa-mir-200c_2DDSD_s
hsa-mir-200c_2DDSD_s (BBa_K5086110) is formed by the combination of hsa-mir-200c_2DDSD (BBa_K5086020), hsa-mir-200c_target (BBa_K5086001), and two wt (BBa_K5086017) chains. Among them, the sequence at the 5' end of hsa-mir-200c_2DDSD was successively linked to hsa-mir-200c_target and two wt chains in a complementary pair, and the sequence at its 3' end was complementary to the Universalness_h (BBa_K5086009) chain, thus hsa-mir-200c_2DDSD_s can bind specifically to the Universalness_h strand and thus complete the recognition of the input signal. DNA polymerase polymerizes DNA in the 5'-3' direction starting from the 3' end of the target hsa-mir-200c_Rep. During DNA polymerization by Bst DNA polymerase, the wt strand is displaced and released by the newly synthesized daughter strand, and a single strand of DNA with the same sequence as the target strand is also released. During the DNA polymerization by Bst DNA polymerase, the wt strand will be replaced and released by the newly synthesized daughter strand, and also release the DNA single strand with the same sequence as that of the target strand (U to T), which will act as a target to undergo the first step of the polymerase-mediated strand replacement reaction again to realize the self-enhancement of the DDSD process, and the wt strand will be involved in the subsequent reaction to produce the fluorescence report.
Sequence and Features
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