Part:BBa_K5086020
hsa-mir-200c_2DDSD
hsa-mir-200c_2DDSD (BBa_K5086020) is a single-stranded DNA sequence with 88 nucleotides that is a self-enhancing probe for the target hsa-mir-200c_Rep. The sequence at its 3' end is designed to be complementary to the Universalness_h strand, so that it binds specifically to the Universalness_h strand and thus accomplishes recognition of the input signal. DNA polymerase polymerizes DNA in the 5'-3' direction starting from the 3' end of the target hsa-mir-200c_Rep. During DNA polymerization by Bst DNA polymerase, the wt strand is displaced and released by the newly synthesized daughter strand, and a single strand of DNA with the same sequence as the target strand is also released. During the DNA polymerization by Bst DNA polymerase, the wt strand will be replaced and released by the newly synthesized daughter strand, and also release the DNA single strand with the same sequence as that of the target strand (U to T), which will act as a target to undergo the first step of the polymerase-mediated strand replacement reaction again to realize the self-enhancement of the DDSD process, and the wt strand will be involved in the subsequent reaction to produce the fluorescence report.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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