Part:BBa_K5086016
Universalness_Q
Universalness_Q (BBa_K5086016) is a 25-nucleotide single-stranded DNA sequence with a 5' modification of the quenching motif BHQ1. Together with the corresponding fluorescent probe Universalness_F (BBa_K5086014) it forms a set of reporter probes. This group of reporter probes is quenched when the target nucleic acid is not present, the reporter probes bring the distance between the fluorescent molecules and the quenching molecules closer due to the formation of a stable double-stranded structure, resulting in fluorescence resonance energy transfer (FRET) occurring, and the quenching of the fluorescence; when the target is added, the wt strand, which is produced by the polymerase mediated strand displacement, hybridizes with the long DNA strand in the double-stranded probe through the toehold mediated strand displacement reaction, resulting in the formation of a thermodynamically more When the target is added, the wt chain from the polymerase-mediated chain replacement hybridizes with the DNA long chain in the double-stranded probe through the toe-mediated chain replacement reaction, resulting in the formation of a thermodynamically more stable double-stranded structure, and at the same time competes with the DNA short chain for fluorescence recovery, releasing fluorescence signal.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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