Protein_Domain

Part:BBa_K5086004:Design

Designed by: Zhaohui Zhang   Group: iGEM24_BUCT-China   (2024-08-28)


Bst DNA Polymerase, Large Fragment


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1468
    Illegal PstI site found at 356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1468
    Illegal PstI site found at 356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 712
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1468
    Illegal PstI site found at 356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 712
    Illegal SpeI site found at 1468
    Illegal PstI site found at 356
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 511


Design Notes

protein


Source

source Bacillus thermophilus with codon optimization. McClary, J., Ye, S. Y., Hong, G. F., & Witney, F. (1991). Sequencing with the large fragment of DNA polymerase I from Bacillus stearothermophilus. DNA Sequence, 1(3), 173-180. Aliotta, J. M., Pelletier, J. J., Ware, J. L., Moran, L. S., Benner, J. S., & Kong, H. (1996). Thermostable Bst DNA polymerase I lacks a 3′→ 5′ proofreading exonuclease activity. Genetic analysis: biomolecular engineering, 12(5-6), 185-195. https://freegenes.github.io/genes/BBF10K_003262.html Source: Open Enzymes Collection, Open Bioeconomy & The FreeGenes Project

References