DNA

Part:BBa_K5082016

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-28)

miR-22-sponge-improvement

Usage and Biology

From 2023 YiYe-China’s results, we found that the miR-22 sensor worked more accurately and efficiently than miR-145. Therefore, we decided to design our improved experiment based on the miR-22 sensor(BBa_K4789002).

This year, we redesigned the miR-22-sponge-pepper part by adding three additional miR-22 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-22 according to a previous study [6]. Therefore, the new sensor will be more sensitive to the miR-22 concentration in cells. The part is called Improved miR-22-sponge-pepper (BBa_K5082017) and its sequence is shown in Figure 1. 

                              improved-1.png
                                Figure 1. Part improved miR-22-sponge-pepper.

References

[1] YiYe-China. “Home | YiYe-China - IGEM 2023.” iGEM.wiki, 2023, 2023.igem.wiki/yiye-china/. Accessed 7 Aug. 2024.

[2] ---. “Cervical Cancer.” World Health Organization, 2019, www.who.int/health-topics/cervical-cancer#tab=tab_1. Accessed 8 Aug. 2024.

[3] JIANG, YAN, et al. “The Role of MALAT1 Correlates with HPV in Cervical Cancer.” Oncology Letters, vol. 7, no. 6, 24 Mar. 2014, pp. 2135–2141, https://doi.org/10.3892/ol.2014.1996. Accessed 9 Aug. 2024.

[4] Kazem Nejati, et al. “MicroRNA-22 in Female Malignancies: Focusing on Breast, Cervical, and Ovarian Cancers.” Pathology - Research and Practice, vol. 223, 1 July 2021, pp. 153452–153452, https://doi.org/10.1016/j.prp.2021.153452. Accessed 9 Aug. 2024.

[5] Sawant, Dwitiya, and Brenda Lilly. “MicroRNA-145 Targets in Cancer and the Cardiovascular System: Evidence for Common Signaling Pathways.” Vascular Biology, 23 Oct. 2020, https://doi.org/10.1530/vb-20-0012. Accessed 9 Aug. 2024.

[6] Ebert, Margaret S et al. “MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells.” Nature methods vol. 4,9 (2007): 721-6. doi:10.1038/nmeth1079. Accessed 9 Aug. 2024.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 147
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 147
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 147
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 147
  • 1000
    COMPATIBLE WITH RFC[1000]


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