Composite

Part:BBa_K5082012:Design

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-07)


pCMV-EGFP-EIF3B-MUT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1684
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 270
    Illegal PstI site found at 1689
    Illegal PstI site found at 3043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1684
    Illegal NheI site found at 916
    Illegal SpeI site found at 270
    Illegal PstI site found at 1689
    Illegal PstI site found at 3043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1684
    Illegal BglII site found at 1664
    Illegal BglII site found at 6170
    Illegal BamHI site found at 1715
    Illegal XhoI site found at 1668
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1684
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 270
    Illegal PstI site found at 1689
    Illegal PstI site found at 3043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1684
    Illegal XbaI site found at 1721
    Illegal SpeI site found at 270
    Illegal PstI site found at 1689
    Illegal PstI site found at 3043
    Illegal NgoMIV site found at 2153
    Illegal NgoMIV site found at 3494
    Illegal NgoMIV site found at 3777
    Illegal AgeI site found at 925
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 903
    Illegal BsaI.rc site found at 5302
    Illegal SapI site found at 4222
    Illegal SapI.rc site found at 3343
    Illegal SapI.rc site found at 3553


Design Notes

1


Source

1

References