Regulatory

Part:BBa_K5082007

Designed by: Fangyuan Duan   Group: iGEM24_YiYe-China   (2024-09-07)

Ras-GTPase-activating protein SH3 domain binding protein 1

Usage and Biology

Ras-GTPase-activating protein SH3 domain binding protein 1 is a cytoplastic multifunctional binding protein [1]. It is involved in many biological reactions including RNA metabolism, cell proliferation, apoptosis, metastasis, differentiation. Regarding the role of G3BP1 in RNA metabolism, it can bind with highly structured 3’ UTR (HSU) mRNA and recruit proteins to degrade the HSU mRNA [1]. Therefore, G3BP1 is an effective regulator in expression of genes with HSU mRNA. The mechanism of G3BP1 on HSU mRNA is shown in Figure 1. 

                            g3bp1.png
                    Figure 1. G3BP1 binds to HSU mRNA and facilitates degradation.

Design G3BP1 plays important roles in multiple diseases and is considered a potential biomarker. In cancer cells, G3BP1’s roles include promoting stress granule assembly and affecting ribosomal stability [2]. To our interest, upregulated G3BP1, and subsequently higher stress granule levels, activates the TGF-β/Smad signaling pathway can promote gastric cancer (GC). Therefore, G3BP1 can be a potential biomarker for GC. In our experiment, we utilized the HSU mRNA-degrading activity of G3BP1. By connecting HSU mRNA sequences downstream reporter genes, reporter protein expression levels reflected G3BP1 concentrations and thus enables GC diagnosis.


Reference

Reference

[1] Ge, Yidong et al. “The roles of G3BP1 in human diseases (review).” Gene vol. 821 (2022): 146294. doi:10.1016/j.gene.2022.146294

[2] Sidibé, Hadjara et al. “The multi-functional RNA-binding protein G3BP1 and its potential implication in neurodegenerative disease.” Journal of neurochemistry vol. 157,4 (2021): 944-962. doi:10.1111/jnc.15280

[3] Xiong, Rui et al. “G3BP1 activates the TGF-β/Smad signaling pathway to promote gastric cancer.” OncoTargets and therapy vol. 12 7149-7156. 2 Sep. 2019, doi:10.2147/OTT.S213728



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 704
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 704
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1244
    Illegal XhoI site found at 1301
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 704
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 704
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None