Coding

Part:BBa_K5079030:Design

Designed by: Marianna Florez   Group: iGEM24_Queens-Canada   (2024-09-28)


Protein Switch 5 (K_45_WT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 785
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part's insertion site has a free energy of 1.1. The insertion's residue has a SASA of 57%. The residue is conserved. This protein switch has not gone through circular permutation. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 16 and the loop distance is 20.6. The linker itself is 3 residues long, composed of "GPG".


Source

References