Coding

Part:BBa_K5079029

Designed by: Marianna Florez   Group: iGEM24_Queens-Canada   (2024-09-28)


Protein Switch 4 (N14__CP_N114)

This part's insertion site has a free energy of 1.0. The insertion's residue has a SASA of 83%. The residue is somewhat similar; not being conserved. This protein switch has gone through circular permutation, and it has a Catalytic Triad RMSD of 0.048. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 2.9 and the loop distance is 5.2. The linker itself is 3 residues long, composed of "GPG".


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None