Coding

Part:BBa_K5078008

Designed by: Elise Bennett   Group: iGEM24_UVU-Utah-2   (2024-09-28)


pL0 Psr1

Phosphate starvation response 1 (Psr1) gene from Chlamydomonas reinhardtii. This gene codes for a transcription factor that activates increased phosphate uptake and storage. It is normally expressed under conditions of low phosphate concentration. When expressed constitutively, it should cause high rates of phosphate uptake regardless of phosphate concentrations in the environment.

This part was obtained from the Alison Baker Lab at the University of Leeds, as published in Slocombe et al. (2023).


Figure 1. Psr1-HA in a level 0 plasmid for later golden gate assembly.

Assembly verification


Figure 2. BsaI digest generates two fragments of the expected sizes.

Structure modeling


Figure 3. Protein modeling of the PSR1 protein.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 80
    Illegal PstI site found at 567
    Illegal PstI site found at 776
    Illegal PstI site found at 946
    Illegal PstI site found at 1423
    Illegal PstI site found at 1858
    Illegal PstI site found at 2026
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1592
    Illegal NheI site found at 2348
    Illegal PstI site found at 80
    Illegal PstI site found at 567
    Illegal PstI site found at 776
    Illegal PstI site found at 946
    Illegal PstI site found at 1423
    Illegal PstI site found at 1858
    Illegal PstI site found at 2026
    Illegal NotI site found at 2811
    Illegal NotI site found at 2901
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 80
    Illegal PstI site found at 567
    Illegal PstI site found at 776
    Illegal PstI site found at 946
    Illegal PstI site found at 1423
    Illegal PstI site found at 1858
    Illegal PstI site found at 2026
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 80
    Illegal PstI site found at 567
    Illegal PstI site found at 776
    Illegal PstI site found at 946
    Illegal PstI site found at 1423
    Illegal PstI site found at 1858
    Illegal PstI site found at 2026
    Illegal NgoMIV site found at 800
    Illegal NgoMIV site found at 1168
    Illegal NgoMIV site found at 1201
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1293
    Illegal NgoMIV site found at 1312
    Illegal NgoMIV site found at 1338
    Illegal AgeI site found at 99
    Illegal AgeI site found at 1186
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/transcriptionalregulator
Parameters
None