Coding

Part:BBa_K5077018

Designed by: Boy Hansen, Malcolm Summer Rose-Heine   Group: iGEM24_Hamburg   (2024-09-28)


EGFP + Linker + Overhangs

This part was developed for the purpose of homologous recombination of pUC-HasA (BBa_K5077015, BBa_K5077014) and EGFP (BBa_J428076). The objective was to observe the production of hyaluronic acid synthase within the cells. Consequently, we attempted a process of homologous recombination, which necessitated the inclusion of the EGFP gene with overhangs. The linker was inserted to facilitate the correct folding of HasA and only acts as a spacer. The overhangs represent the pUC-HasA vector. The overhangs represent the exact position where the EGFP is to be inserted. As a result, this part has overlapping sequences with the pUC-HasA vector, which is important for homologous recombination. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None