Plasmid_Backbone
Part:BBa_K5077017
Designed by: Katharina Veith Group: iGEM24_Hamburg (2024-09-28)
pET-28c(+)
The plasmid backbone of the pET-28c(+) vector is illustrated here. The pET-28c(+) vector is similar to the pET28a(+) vector which is a frequently used expression vector in E. coli. The plasmid backbone contains a T7 promoter that can be controlled with precision via IPTG. To facilitate the purification of the protein, the vector incorporates a His-tag and thrombin- and enterokinase restriction sites, which can be employed to remove the His-tag following the purification process. The kanamycin resistance enables the differentiation between transformed and non-transformed cells.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5030 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 5153 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BglII site found at 4964
Illegal XhoI site found at 5162 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5030 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 5030
Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 3184
Illegal NgoMIV site found at 3344
Illegal NgoMIV site found at 4932 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 2263
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Categories
Parameters
None |