Plasmid_Backbone

Part:BBa_K5077017

Designed by: Katharina Veith   Group: iGEM24_Hamburg   (2024-09-28)


pET-28c(+)

The plasmid backbone of the pET-28c(+) vector is illustrated here. The pET-28c(+) vector is similar to the pET28a(+) vector which is a frequently used expression vector in E. coli. The plasmid backbone contains a T7 promoter that can be controlled with precision via IPTG. To facilitate the purification of the protein, the vector incorporates a His-tag and thrombin- and enterokinase restriction sites, which can be employed to remove the His-tag following the purification process. The kanamycin resistance enables the differentiation between transformed and non-transformed cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5030
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NotI site found at 5153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 4964
    Illegal XhoI site found at 5162
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5030
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 5030
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI.rc site found at 2263


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Categories
Parameters
None