Plasmid

Part:BBa_K5077016

Designed by: Boy Hansen, Malcolm Summer Rose-Heine, Lena Abeln   Group: iGEM24_Hamburg   (2024-09-28)


pUC_hasA_EGFP

The plasmid may be employed for the expression of a hyaluronic acid synthase fusion protein with enhanced green fluorescent protein (EGFP) in E. coli cells.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5185
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5185
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5185
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5185
    Illegal AgeI site found at 3724
    Illegal AgeI site found at 4330
    Illegal AgeI site found at 4366
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1351
    Illegal SapI site found at 268
    Illegal SapI site found at 3706


[edit]
Categories
Parameters
None