Plasmid
Part:BBa_K5077016
Designed by: Boy Hansen, Malcolm Summer Rose-Heine, Lena Abeln Group: iGEM24_Hamburg (2024-09-28)
pUC_hasA_EGFP
The plasmid may be employed for the expression of a hyaluronic acid synthase fusion protein with enhanced green fluorescent protein (EGFP) in E. coli cells.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 5185
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 5185
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3889
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 5185
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 5185
Illegal AgeI site found at 3724
Illegal AgeI site found at 4330
Illegal AgeI site found at 4366 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1351
Illegal SapI site found at 268
Illegal SapI site found at 3706
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Categories
Parameters
None |