Plasmid_Backbone
Part:BBa_K5077015
Designed by: Lena Abeln Group: iGEM24_Hamburg (2024-09-28)
pUC with araC and AmpR
The plasmid backbone is suitable for use in a number of different cloning approaches. The ampicillin resistance enables the differentiation between transformed and non-transformed cells, while the araC site facilitates the regulation of gene expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BamHI site found at 3889 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal AgeI site found at 3724 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1351
Illegal SapI site found at 268
Illegal SapI site found at 3706
[edit]
Categories
Parameters
None |