Plasmid_Backbone

Part:BBa_K5077015

Designed by: Lena Abeln   Group: iGEM24_Hamburg   (2024-09-28)


pUC with araC and AmpR

The plasmid backbone is suitable for use in a number of different cloning approaches. The ampicillin resistance enables the differentiation between transformed and non-transformed cells, while the araC site facilitates the regulation of gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 3889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal AgeI site found at 3724
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1351
    Illegal SapI site found at 268
    Illegal SapI site found at 3706


[edit]
Categories
Parameters
None