Plasmid_Backbone

Part:BBa_K5077007

Designed by: AG Deuerling; University of Constance   Group: iGEM24_Hamburg   (2024-09-27)


pMK1

The plasmid was generated by a research group at the University of Constance. This plasmid was selected for the straightforward generation of multiple copies of the resilin gene (BBa_K5077000) via type II restriction enzymes. The plasmid has a single restriction site for each of the enzymes type II (BsaI, BsmBI, SacI).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 386
    Illegal XhoI site found at 638
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 364
    Illegal BsaI.rc site found at 2010
    Illegal SapI site found at 927
    Illegal SapI site found at 3031


[edit]
Categories
Parameters
None