Plasmid_Backbone
Part:BBa_K5077007
Designed by: AG Deuerling; University of Constance Group: iGEM24_Hamburg (2024-09-27)
pMK1
The plasmid was generated by a research group at the University of Constance. This plasmid was selected for the straightforward generation of multiple copies of the resilin gene (BBa_K5077000) via type II restriction enzymes. The plasmid has a single restriction site for each of the enzymes type II (BsaI, BsmBI, SacI).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 386
Illegal XhoI site found at 638 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 364
Illegal BsaI.rc site found at 2010
Illegal SapI site found at 927
Illegal SapI site found at 3031
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Categories
Parameters
None |