Coding

Part:BBa_K5077003:Design

Designed by: Luna Brandes, Greta Stoltmann, Boy Hansen   Group: iGEM24_Hamburg   (2024-09-24)


Resilin Oligo NdeI 1 end


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon usage was optimised and any undesirable restriction sites were removed. To this Oligonucleotide a restriction site for NdeI was added to help with the insertion into the vector later on. There are also a few added base pairs to create a landing strip for the restriction enzymes.


Source

The original gene sequence was derived from the organism Drosophila melanogaster.

References