Coding
Part:BBa_K5077002:Design
Designed by: Luna Brandes, Greta Stoltmann, Boy Hansen Group: iGEM24_Hamburg (2024-09-24)
Resilin Oligo 2 repeat
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The codon usage was optimised and any undesirable restriction sites were removed. The complete Resilin exon optimized sequence (BBa_K5077000) was divided by half and put together with the end in front and the beginning behind that. We therefore have achieved that the first and the second Oligonucleotide bind together with long overhangs on both sides.
Source
The original gene sequence was derived from the organism Drosophila melanogaster.