Coding

Part:BBa_K5077002:Design

Designed by: Luna Brandes, Greta Stoltmann, Boy Hansen   Group: iGEM24_Hamburg   (2024-09-24)


Resilin Oligo 2 repeat


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon usage was optimised and any undesirable restriction sites were removed. The complete Resilin exon optimized sequence (BBa_K5077000) was divided by half and put together with the end in front and the beginning behind that. We therefore have achieved that the first and the second Oligonucleotide bind together with long overhangs on both sides.

Source

The original gene sequence was derived from the organism Drosophila melanogaster.

References