Part:BBa_K5073010

GP3C scFv

GPC3 scFv (single-chain variable fragment) is a genetically engineered antibody fragment designed to specifically recognize the glypican-3 (GPC3) antigen, which is overexpressed in hepatocellular carcinoma (HCC) and other cancers. This scFv consists of the variable regions of both the heavy and light chains (VH and VL) connected by a flexible linker. It is typically used in CAR-T cell therapy, where it functions as the antigen-binding domain of the chimeric antigen receptor (CAR), allowing T cells to specifically recognize and attack GPC3-positive cancer cells.

To use this part in your project, you can clone the GPC3 scFv gene into a suitable vector for expression in your system, typically in CAR-T cells or other immune cell-based therapies. It's essential to ensure that your expression system is optimized for proper folding and surface display of the scFv. This part can be integrated into various therapeutic or diagnostic platforms targeting GPC3-expressing tumors.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]