Part:BBa_K5071021

pETDuet-1 backbone

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NotI site found at 149
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 305
  Illegal BamHI site found at 106
  Illegal XhoI site found at 354
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NgoMIV site found at 324
  Illegal NgoMIV site found at 671
  Illegal NgoMIV site found at 5348
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

BBa_K5071021 (pETDuet-1 backbone)

Name

pETDuet-1 backbone

Base Pairs

5178 bp

Origin

Escherichia coli

Usage and Biology

The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, and we used this vector for the connection of the target genes.

Cultivation

The pETDuet-1 backbone is a plasmid vector obtained by linearizing the plasmid pETD, with a length of 5178 bp. Fig 1. shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid.

Fig 1. The purpose segment of backbone pETDuet-1