Part:BBa_K5071010

BGCII-5

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 700
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 700
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 700
  Illegal BglII site found at 1131
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 700
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 700
  
• 1000
  COMPATIBLE WITH RFC[1000]

Composite part BBa_K5071010 (BGCII-5)

Name: BGCII-5

Base Pairs: 1500 bp

Origin: Paceibacterales

Usage and Biology

BGCII-5 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCII-5 gene, with a length of 1500 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pRSFuet-BGCII-gene685.

Fig 1. The purpose segment of plasmid pRSFuet-BGCII-gene685