Part:BBa_K5071009

BGCII-4

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 56
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

<!DOCTYPE html> BBa_K5071009 (BGCII-4)

Composite part BBa_K5071009 (BGCII-4)

Name: BGCII-4

Base Pairs: 150 bp

Origin: Paceibacterales

Usage and Biology

BGCII-4 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCII-4 gene, with a length of 150 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pACYCDuet-BGCII-gene143.

Fig 1. The purpose segment of plasmid pACYCDuet-BGCII-gene143