Part:BBa_K5071003

BGCI-4

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 520
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 520
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 378
  Illegal BamHI site found at 390
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 520
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 520
  
• 1000
  COMPATIBLE WITH RFC[1000]

Composite part BBa_K5071003 (BGCI-4)

Name: BGCI-4

Base Pairs: 1300 bp

Origin: Bacteriovoracaceae

Usage and Biology

BGCI-4 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCI-4 gene, with a length of 1300 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene456.

Fig 1. The purpose segment of plasmid pRSFuet-BGC1-gene456