Composite

Part:BBa_K5066009

Designed by: Abner Tseng   Group: iGEM24_GEMS-Taiwan   (2024-09-21)

P20-Cyt2Ba-BmK IT1

Description

Insecticide resistance is becoming increasingly problematic and prominent in many Southeast Asian countries, areas where dengue fever is prevalent. The combination of Bt toxins and scorpion toxins is a promising strategy providing a potential layer of protection against insecticide resistance. The ribosome binding site(RBS) is a sequence of nucleotides before the start codon of an mRNA transcript and functions to recruit ribosomes for the translation of proteins. The His-tag, 6X His, is a commonly used purification tag that contains 6 consecutive histidine residues. The His-tag can be stained with His-tag antibodies after translation, labelling the target recombinant protein. It is typically placed on either the N or C terminus of a protein.

Use in Biology

P20 is a chaperone that aids in the production of the Bt toxins. Cyt2ba is a Bt toxin that binds to membrane receptors and increases membrane permeability. In Aedes mosquitoes, the toxin binds to the midgut of the mosquito larvae. Due to its crystalline structure, the toxin alters the cell membrane’s permeability which is crucial for cellular transport and activities, ultimately leading to the death of the larvae as a result of malnutrition and damage to the cell membrane. BmK TI1 is a scorpion toxin that acts as a neurotoxin, and it is a type of toxin that is selectively active on lepidopterous and dipterous insects. Due to its high insect toxicity and its selectivity for only lepidopterous and dipterous insects, scorpion toxins can be applied as potential biological insecticides in pest control.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1038
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1038
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1038
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1038
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1038
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference

Yu-Mei Xiong, Min-Hua Ling, Zheng-Dao Lan, Da-Cheng Wang, Cheng-Wu Chi,The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch,Toxicon, Volume 37, Issue 2, 1999, Pages 335-341, ISSN 0041-0101, https://doi.org/10.1016/S0041-0101(98)00176-7.

Deng, S. Q., Deng, M. Z., Chen, J. T., Zheng, L. L., & Peng, H. J. (2017). Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 37(6), 750–754. https://doi.org/10.3969/j.issn.1673-4254.2017.06.06

Bravo, A., Likitvivatanavong, S., Gill, S. S., & Soberón, M. (2011). Bacillus thuringiensis: A story of a successful bioinsecticide. Insect biochemistry and molecular biology, 41(7), 423–431. https://doi.org/10.1016/j.ibmb.2011.02.006

Gu, J. B., Dong, Y. Q., Peng, H. J., & Chen, X. G. (2010). A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus. The American journal of tropical medicine and hygiene, 83(3), 614–623. https://doi.org/10.4269/ajtmh.2010.10-0074

[edit]
Categories
Parameters
None