Composite

Part:BBa_K5066008:Design

Designed by: Abner Tseng   Group: iGEM24_GEMS-Taiwan   (2024-09-21)


P20-Cyt2Ba


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1039
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1039
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1039
    Illegal BglII site found at 777
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1039
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 264
    Illegal EcoRI site found at 1039
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

P20 or 20-kilodalton protein is an accessory protein in Bt strains. P20 is a chaperone protein in Bt strains, and it serves to increase yields and facilitates crystal formation of various insecticidal crystal proteins by helping with the folding and unfolding of the protein structure. The presence of P20 ensures the quality of the proteome expressed, in this case, Bt proteins, by stabilizing, interacting with, and remodeling the polypeptides. We combined Cyt2ba with the booster P20 to ensure that the protein folds properly. The P20 booster attaches to the fragment sequence to help facilitate the folding and formation of the protein structure which can increase the concentration of our product.

Source

Engineered by GEMS Academy

References

Shi, Yongxia, et al. “Interactions between Molecular Chaperone P20 and Cyt2Ba7 Toxin inBacillus Thuringiensis.” BioRxiv (Cold Spring Harbor Laboratory), 27 Apr. 2017, https://doi.org/10.1101/129833. https://www.biorxiv.org/content/10.1101/129833v1.full

Yu-Mei Xiong, Min-Hua Ling, Zheng-Dao Lan, Da-Cheng Wang, Cheng-Wu Chi,The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch,Toxicon, Volume 37, Issue 2, 1999, Pages 335-341, ISSN 0041-0101, https://doi.org/10.1016/S0041-0101(98)00176-7.

Deng, S. Q., Deng, M. Z., Chen, J. T., Zheng, L. L., & Peng, H. J. (2017). Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 37(6), 750–754. https://doi.org/10.3969/j.issn.1673-4254.2017.06.06

Bravo, A., Likitvivatanavong, S., Gill, S. S., & Soberón, M. (2011). Bacillus thuringiensis: A story of a successful bioinsecticide. Insect biochemistry and molecular biology, 41(7), 423–431. https://doi.org/10.1016/j.ibmb.2011.02.006