Part:BBa_K5066006:Design
BmK IT1-Cyt2Ba
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 733
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 733
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 733
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 733
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 733
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
BmK is a neurotoxin, and Cyt2ba is a Bt toxin, and the combination of the two types of toxin can help prevent the possibility of insecticide resistance.
Source
Engineered in GEMS Academy
References
Gu, J. B., Dong, Y. Q., Peng, H. J., & Chen, X. G. (2010). A recombinant AeDNA containing the insect-specific toxin, BmK IT1, displayed an increasing pathogenicity on Aedes albopictus. The American journal of tropical medicine and hygiene, 83(3), 614–623. https://doi.org/10.4269/ajtmh.2010.10-0074
Deng, S. Q., Deng, M. Z., Chen, J. T., Zheng, L. L., & Peng, H. J. (2017). Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 37(6), 750–754. https://doi.org/10.3969/j.issn.1673-4254.2017.06.06
Yu-Mei Xiong, Min-Hua Ling, Zheng-Dao Lan, Da-Cheng Wang, Cheng-Wu Chi, The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch,Toxicon,Volume 37,Issue 2,1999,Pages 335-341,ISSN 0041-0101,https://doi.org/10.1016/S0041-0101(98)00176-7.
Yu-Mei Xiong, Min-Hua Ling, Zheng-Dao Lan, Da-Cheng Wang, Cheng-Wu Chi,The cDNA sequence of an excitatory insect selective neurotoxin from the scorpion Buthus martensi Karsch,Toxicon, Volume 37, Issue 2, 1999, Pages 335-341, ISSN 0041-0101, https://doi.org/10.1016/S0041-0101(98)00176-7.
Bravo, A., Likitvivatanavong, S., Gill, S. S., & Soberón, M. (2011). Bacillus thuringiensis: A story of a successful bioinsecticide. Insect biochemistry and molecular biology, 41(7), 423–431. https://doi.org/10.1016/j.ibmb.2011.02.006