Part:BBa_K5066005
Xpp81Aa1-BmK IT1
Description
Insecticide resistance is becoming increasingly problematic and prominent in many Southeast Asian countries, areas where dengue fever is prevalent. The combination of Bt toxins and scorpion toxins is a promising strategy providing a potential layer of protection against insecticide resistance. The ribosome binding site(RBS) is a sequence of nucleotides before the start codon of an mRNA transcript and functions to recruit ribosomes for the translation of proteins. The His-tag, 6X His, is a commonly used purification tag that contains 6 consecutive histidine residues. The His-tag can be stained with His-tag antibodies after translation, labelling the target recombinant protein. It is typically placed on either the N or C terminus of a protein.
Usage and Biology
Xpp81Aa1 is a Bt toxin that is used as an insecticide as it can target specific insects without causing harm to other species. There are a wide variety of strains derived from a selection of Bt bacteria and each has similar effects but targets different species of insects. There are three main categories of the Bt toxin: Cry, Cyt, and Vip; there are also the Xpp, which were renamed from Cry.[1] BmK TI1 is a scorpion toxin that acts as a neurotoxin, and it is a type of toxin that is selectively active on lepidopterous and dipterous insects.[2] Due to its high insect toxicity and its selectivity for only lepidopterous and dipterous insects, scorpion toxins can be applied as potential biological insecticides in pest control.
Table 1. The mortality rate of Aedes albopictus larvae exposed to various combinations of biolarvicidal toxins.
Our results indicated that combining two or three biolarvicidal toxins achieved a maximum mortality rate of only 33.33%. The limited effectiveness observed in this study highlights the need for further research to optimize for more effective toxin combinations and formulations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
[1] Shilling, P. J., Mirzadeh, K., Cumming, A. J., Widesheim, M., Köck, Z., & Daley, D. O. (2020). Improved designs for pET expression plasmids increase protein production yield in Escherichia coli. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-0939-8
[2] Deng, S.-Q., Deng, M.-Z., Chen, J.-T., Zheng, L.-L., & Peng, H.-J. (2017). [Larvicidal activity of recombinant Escherichia coli expressing scorpion neurotoxin AaIT or B.t.i toxin Cyt2Ba against mosquito larvae and formulations for enhancing the effects]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 37(6). https://doi.org/10.3969/j.issn.1673-4254.2017.06.06
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