RBS

Part:BBa_K5062038

Designed by: Soumik Ojha   Group: iGEM24_HKU-HongKong   (2024-09-27)


Internal Ribosome Entry Site (IRES)

Description: Internal ribosome entry site (IRES) is derived from alphaviruses [Kamrud, 2007]. It enables 5’-cap independent translation of downstream genes [Kamrud, 2007].


Usage and Biology

This part is useful in creation of bicistronic or polycistronic protein expression systems, when cloned upstream of genes.

References

Kamrud, K. I., Custer, M., Dudek, J. M., Owens, G., Alterson, K. D., Lee, J. S., Groebner, J. L., & Smith, J. F. (2007). Alphavirus replicon approach to promoterless analysis of IRES elements. Virology, 360(2), 376–387. https://doi.org/10.1016/j.virol.2006.10.049 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None