Part:BBa_K5062031

SARS-CoV-2 Proofreading Cofactor (nsP10)

Gene coding for protein nsP10, a nonstructural protein originating from Coronaviruses [1]. It acts as an activating cofactor to nsP14 [1]. A complete nsP14-nsP10 complex acts as a proofreading exonuclease, and decreases transcription mutational error rates from self-amplification of RNA [1]. Usage and Biology: This part is useful in providing proofreading abilities to increase expression of a system by decreasing transcription mutation rates. It should be paired with nsP14 to form the complete complex to enable this ability. When implemented into a construct, nsP10 should be cloned between nsP3 and nsP4.

Usage and Biology

This part is useful in providing proofreading abilities to increase expression of a system by decreasing transcription mutation rates. It should be paired with nsP14 to form the complete complex to enable this ability. When implemented into a construct, nsP10 should be cloned between nsP3 and nsP4.

References

1. Rona, G., Zeke, A., Miwatani-Minter, B. et al. The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target. Cell Death Differ 29, 285–292 (2022). https://doi.org/10.1038/s41418-021-00900-1 Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

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