Part:BBa_K5062029

No part name specified with partinfo tag.

Sequence and Features No part name specified with partinfo tag.

Overview

Gene coding for protein nsP14, a nonstructural protein originating from Coronaviruses [7]. It is a bifunctional dsRNA exonuclease/guanosine 5’ methyltransferase protein, and acts as the catalytic subunit of the nsP14-nsP10 complex [5]. A complete nsP14-nsP10 complex acts as a proofreading exonuclease, and decreases mutational error rates from self-amplification of RNA [7].

Usage and Biology

This part is useful in providing proofreading abilities to increase expression of a system by decreasing mutation rates. It should be paired with nsP10 to form the complete complex to enable this ability. When implemented into a construct, nsP14 should be cloned downstream of nsP4, upstream of 26S/-26S subgenomic promoters.

References

Rona, G., Zeke, A., Miwatani-Minter, B. et al. The NSP14/NSP10 RNA repair complex as a Pan-coronavirus therapeutic target. Cell Death Differ 29, 285–292 (2022). https://doi.org/10.1038/s41418-021-00900-1

Kamrud, K. I., Custer, M., Dudek, J. M., Owens, G., Alterson, K. D., Lee, J. S., Groebner, J. L., & Smith, J. F. (2007). Alphavirus replicon approach to promoterless analysis of IRES elements. Virology, 360(2), 376–387. https://doi.org/10.1016/j.virol.2006.10.049