DNA
Part:BBa_K5061215:Design
Designed by: Florent Cha Group: iGEM24_Evry-Paris-Saclay (2024-09-25)
∆ung repair DNA
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 731
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 731
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 731
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 731
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
see main page of this part
Source
PCR aplification from E. coli MG1655* ∆flu ∆ung ∆nfi ∆pyrF described by Álvarez et al., 2020.
References
Álvarez B, Mencía M, de Lorenzo V, Fernández LÁ. In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9. Nature Communications (2020) 11: 6436.