Composite

Part:BBa_K5061056:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Test Plasmid (TP) expressing sfGFP-LVAtag from the Pm promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3099
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3099
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3099
    Illegal AgeI site found at 139
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

DNA synthesis, PCR, Restriction Digest and assembly

References