Composite

Part:BBa_K5061033:Design

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-14)


Expression Plasmid (EP) Golden Gate cloning platform


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 47
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1752
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 53
    Illegal BsaI.rc site found at 41


Design Notes

Source

DNA synthesis, PCR amplifications and Golden Gate assembly

References