Part:BBa_K5061016

nfi gRNA

This part is a guide RNA (gRNA) specific for the nfi gene of E. coli.

Usage and Biology

It was designed using the CHOPCHOP (version 3) web tool for selecting target sites for CRISPR/Cas9 [1–3] and selected as the best ranked among 95 proposed for knocking-out this gene in E. coli str. K-12/MG1655.

The predicted features of this gRNA are:

Target sequence: GATGTCGGGTTTGAGCAGGGCGG
Genomic location: NC_000913.3:4198892
Strand: +
GC content (%): 60%
Self-complementarity: 0
MM0: 1
MM1: 0
MM2: 0
MM3: 0
Efficiency: 81.41

References

[1] Labun K, Montague TG, Krause M, Torres Cleuren YN, Tjeldnes H, Valen E. CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing. Nucleic Acids Research (2019) 47: W171–W174.

[2] Labun K, Montague TG, Gagnon JA, Thyme SB, Valen E. CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering. Nucleic Acids Research (2016) 44: W272-276.

[3] Montague TG, Cruz JM, Gagnon JA, Church GM, Valen E. CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing. Nucleic Acids Research (2014) 42: W401-407.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]