Part:BBa_K5061010

PspA promoter

This part is the promoter of the phage shock protein A (pspA) gene of E. coli.

Usage and Biology

The PpspA promoter is actually bidirectional as it contains, in reverse direction, the promoter of the phage shock protein F (pspF) gene of E. coli. This pspA/pspF promoter is induced by stress factors and is regulated by a network of positive and negative mechanisms [Weiner et al., 1991; Elderkin et al., 2002].

References

Weiner L, Brissette JL, Model P. Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on sigma 54 and modulated by positive and negative feedback mechanisms. Genes Dev. 1991 Oct;5(10):1912-23.

Elderkin S, Jones S, Schumacher J, Studholme D, Buck M. Mechanism of action of the Escherichia coli phage shock protein PspA in repression of the AAA family transcription factor PspF. J Mol Biol. 2002 Jun 28;320(1):23-37.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 71
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]