Composite

Part:BBa_K5055004

Designed by: Laurine Wachspress   Group: iGEM24_Ionis-Paris   (2024-09-30)


Tobacco Mosaic Virus coat protein composite part

In order to inhibit the infection of the Beet Yellows Virus in sugar beets, we designed RNA interference precursors to apply exogenously on the plants. Since RNA is fragile, we planned to protect it by encapsulating it into a viral capsid. Therefore, this part encodes the Tobacco Mosaic Virus coat protein. It contains a T7 promoter and terminator to achieve a high expression of the protein of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 96
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 30
    Illegal BamHI site found at 642
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 96
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 96
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 581


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Categories
Parameters
None