Part:BBa_K5054002

MTS1- Saccharomyces cerevisiae mitochondrial transit peptide

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Profile

Name: MTS1
Base Pairs: 75 bp
Origin: Saccharomyces cerevisiae
Properties: Mitochondrial transit peptide

Usage and Biology

As the mitochondrial genome is very small in size, most proteins present in the organelle are encoded in the nucleus, translated on the cytoplasm and imported into the mitochondria. These proteins are tagged on the N-terminus with mitochondrial transit peptides like this one, which target the protein to the mitochondria. This insert can be taken advantage of to direct proteins to the mitochondria for any type of study on the organelle.

Expression of MTS1-tagged proteins

S. cerevisiae CEN.PK113-5D was transformed to express ymNeongreen with MTS1 concatenated at its N-terminus. Following up on this, liquid cultures of the yeast were collected at exponential phase and imaged under a KNIC spinning disc confocal microscope.

Figure 1. MTS1-mNeongreen yeast transformants under fluorescent confocal microscope at 1000x magnification. The clustering of fluorescence is visible and visually very similar to mitochondrial localization reported by [1].

References

[1] Kunová, N., Ondrovičová, G., Bauer, J. A., Bellová, J., Ambro, Ľ., Martináková, L., Kotrasová, V., Kutejová, E., & Pevala, V. (2017). The role of Lon-mediated proteolysis in the dynamics of mitochondrial nucleic acid-protein complexes. Scientific Reports, 7(1). https://doi.org/10.1038/s41598-017-00632-8