Tag

Part:BBa_K5054001:Design

Designed by: José Ángel Fernández   Group: iGEM24_TU-Delft   (2024-07-22)


uTP2 - UCYN-A transit peptide motif combination 21634


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transit peptide might be "snipped" out of the protein after import into UCYN-A, much like other transit peptides. This should be taken into account when tagging proteins, so it is not advisable to add other sequences (like a his-tag for purification) downstream from it.


Source

The part is made up of motifs that come from the genome of Braarudosphaera bigelowii. Following up on proteomic analysis performed on UCYN-A and its host this year (DOI: 10.1126/science.ado8571) we found a series of motifs common to proteins imported into UCYN-A and absent from proteins that were not imported into it. The part is conformed of the second most common combination of motifs.


References