Intermediate

Part:BBa_K5051006

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-02)


Kozak

This part acts as a connector for Type II promoter parts in the yeast toolkit by Lee (2015) for inserting a 10bp Kozak sequence in the immediate upstream of the start codon. The Kozak sequence is given by 5’-GAAAGAAAAA, i.e. the 5’ UTR motif with the top 5% in yeast, as described by Dvir et al. (2013). This would allow researchers to enhance recombinant protein expression when the Yeast MoClo system is used, which increases the versatility of this toolkit for wider fields of applications. EcoRV and BglII sites are present for inserting into the Type II plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 26


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