Part:BBa_K5051001

PPTase

PPTase from Aureobasidium pullulans EXF-150 is critical for post-translational modifications (PTMs) of PKS1. The conserved mechanism is that the PPTase transfers a 4'-phosphopantetheinyl (4'-PP) group from Coenzyme A (CoA) to a conserved serine in the acyl carrier (ACP) domains of polyketide synthases (PKS) (Sunbul et al., 2009).

This modification converts PKS1 from the inactive apo-form to the active holo-form, enabling them to participate in downstream reactions. The 4′-PP group acts as a flexible arm that facilitates the transport of acyl or aminoacyl intermediates between the active sites. Without PPTase, PKS1 would be hindered from maximum enzymatic activity (Venkitasubramanian et al., 2006; Xue et al., 2018).

Fig 1. Structure predicted by ESMFold, coloured by pLDDT (Lin et al., 2023)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 924
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 981
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]