Coding

Part:BBa_K5051000

Designed by: Yuk Ho Tsang   Group: iGEM24_PLKNPL-HK   (2024-10-01)


PKS1

The PKS1 gene from Aureobasidium pullulans EXF-150 encodes a highly reduced polyketide synthase (HR-PKS) that plays a vital role in synthesising the fatty acid tail of liamocin. This enzyme is involved in the biosynthesis 3,5-dihydroxydecanoic acid chain (fatty acid part) of Liamocin (Xue et al., 2020). The product of the PKS1 gene is the main building block of the lipid moiety of liamocin. This polyketide synthase is a multidomain enzyme that coordinates a series of reactions that are critical to producing the unique dihydroxy fatty acid chains characteristic of the liamocin molecule.

partsnew1.png

Fig 1. Structure predicted by ColabFold, coloured by pLDDT (Jumper et al., 2021; Mirdita et al., 2022)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1012
    Illegal BglII site found at 1504
    Illegal BglII site found at 4192
    Illegal BglII site found at 5371
    Illegal BglII site found at 5677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2939
    Illegal SapI.rc site found at 5579


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Parameters
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