Plasmid

Part:BBa_K5048021:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


plasmid QEP_GST


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6301
    Illegal NotI site found at 6326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6301
    Illegal BglII site found at 4964
    Illegal BamHI site found at 6295
    Illegal XhoI site found at 6335
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6301
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
    Illegal AgeI site found at 5409
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Design Notes

We fuse QEP with FST for further verification.


Source

The insertion fragment is complete synthesis . The backbone is purchased from Genscript.


References