Composite

Part:BBa_K5047043:Design

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-10-01)


Sequence encodes pCYC1 promoter and mCherry for expression in S. cerevisiae.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 39
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 640
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

The pCYC1 promoter originates from the pDA120 plasmid and the mCherry reporter originates from the pDA95 plasmid, both plasmids were provided by Prof. Serge Pelet (DMF, University of Lausanne).


References