Composite

Part:BBa_K5047043

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-10-01)


Sequence encodes pCYC1 promoter and mCherry for expression in S. cerevisiae.

As the pCYC1 constitutive promoter was already characterised, this composite part served as a comparison point to quantify the strength of the pTEF1 promoter..

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 39
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 640
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None