Composite

Part:BBa_K5047042

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-10-01)


Sequence encodes pADH1 promoter and mCherry for expression in S. cerevisiae.

As the pADH1 constitutive promoter was already characterised, this composite part served as a comparison point to quantify the strength of the pTEF1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1797
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1797
    Illegal NgoMIV site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830


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Categories
Parameters
None