Part:BBa_K5047041
Sequence encodes pTEF1 promoter and mCherry for expression in S. cerevisiae
This composite part was created to test the strength of the pTEF1 promoter using an mCherry reporter fluorescence assay.
To evaluate the expression efficiency of the pTEF1 promoter, a plasmid was first constructed where mCherry was placed under the control of pTEF1. The fluorescence intensity resulting from this construct allowed for a direct quantification of the promoters strength, since mCherry expression correlates with the activity of pTEF1.
To benchmark pTEF1s performance, its activity was compared to two other well characterised promoters: pADH1 (BBa_J63005) and pCYC1 (BBa_K124000). Both of these promoters are commonly used, and well documented on the iGEM registry. By comparing the fluorescence intensity of mCherry expression under these three different promoters, it was possible to rank their relative strengths and effectiveness in driving gene expression.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 766
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 766
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 766
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 766
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 160
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