Regulatory

Part:BBa_K5047020

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-09-30)


Constitutive promoter in Lactococcus lactis

This promoter is an endogenous constitutive promoter from L lactis characterized by Ogaugwu et al The author demonstrated that cellobiose enhances activity from PTSIIC promoter and that importantly PTSIIC promoter can mediate protein expression in B subtilis and E coli Nissle 1917 In the context of our project we used the PTSIIC promoter to express shRNA in cells to trigger RNA interference.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None