Coding

Part:BBa_K5043004

Designed by: Richard Meuten   Group: iGEM24_TUDarmstadt   (2024-08-17)


phdF from M. vanbaalenii PYR-1

Encodes ring cleavage dioxygenase presumably catalysing reaction of 4,5-dihydroxypyrene to Phenanthrene-4,5-Dicarboxylate. This is the third step in pyrene biodegradation. This part was derived from Mycobacterium vanbaalenii Pyr-1 which is a known pyrene degrader. [1]

[1] S.-J. Kim, O. Kweon, R. C. Jones, J. P. Freeman, R. D. Edmondson, and C. E. Cerniglia, "Complete and integrated pyrene degradation pathway in Mycobacterium vanbaalenii PYR-1 based on systems biology," Journal of bacteriology, vol. 189, no. 2, pp. 464–472, 2007, doi: 10.1128/jb.01310-06.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 321
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 412
    Illegal AgeI site found at 470
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None