Part:BBa_K5036008
NES
Part Description
It is a Nuclear Export Signal which is a short sequence of amino acids found in certain proteins that direct those proteins out of the nucleus of a cell. . In our model it is engineered in the second chain of dCas9(N)-synVEGFR2 receptor
Usage
It help in increasing control over transcription activity by preventing delivery of dCas9 into the nucleus without activation of our dCas9(N)-synVEGFR2 receptor
this figure illustrates the structure of NES in the second chain of dCas9(N)-synVEGFR2 receptor .
Literature Characterization
Many studies suggest that protein movement between the cell nucleus and cytoplasm (nucleocytoplasmic translocation) is controlled by signals called NLS and NES. While NLS is known to be essential for Mst3 protein entering the nucleus, it's unclear if there's also an NES signal directing full-length Mst3 back out to the cytoplasm. To investigate this possibility, HeLa cells were temporarily modified with a construct expressing EGFP-tagged Mst3 (EGFP-Mst3WT) and then treated with LMB, a drug that blocks a specific protein (Crm1) involved in exporting proteins from the nucleus.
The image reveals that EGFP-Mst3WT, normally found in the cytoplasm, concentrated in the nucleus following LMB treatment. This suggests the presence of a functional NES-like signal in Mst3. This signal might be responsible for exporting the full-length Mst3 protein from the nucleus through a mechanism dependent on the Crm1 protein.
Reference
Lee, W. S., Hsu, C. Y., Wang, P. L., Huang, C. Y. F., Chang, C. H., & Yuan, C. J. (2004). Identification and characterization of the nuclear import and export signals of the mammalian Ste20‐like protein kinase 3. FEBS letters, 572(1-3), 41-45.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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